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  1. Methods

    Generation of a luciferase-based reporter for CHH and CG DNA methylation inArabidopsis thaliana

    DNA methylation ensures genome integrity and regulates gene expression indiverse eukaryotes. In Arabidopsis, methylation occurs in threesequence contexts: CG, CHG and CHH. The initial establishment of DNAmethylat...

    Thanh Theresa Dinh, Michael O’Leary, So Youn Won, Shengben Li, Lorena Arroyo, Xigang Liu, Andrew Defries, Binglian Zheng, Sean R Cutler and Xuemei Chen

    Silence 2013 4:1

    Published on: 5 April 2013

  2. Research

    MicroRNAs from the same precursor have different targeting properties

    The processing of a microRNA results in an intermediate duplex of two potential mature products that derive from the two arms (5′ and 3′) of the precursor hairpin. It is often suggested that one of the sequenc...

    Antonio Marco, Jamie I MacPherson, Matthew Ronshaugen and Sam Griffiths-Jones

    Silence 2012 3:8

    Published on: 27 September 2012

  3. Methods

    Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis thaliana

    Cytosine methylation is an important chromatin modification that maintains genome integrity and regulates gene expression through transcriptional gene silencing. Major players in de novo methylation guided by siR...

    So Youn Won, Shengben Li, Binglian Zheng, Yuanyuan Zhao, Dongming Li, Xin Zhao, Huilan Yi, Lei Gao, Thanh Theresa Dinh and Xuemei Chen

    Silence 2012 3:6

    Published on: 7 June 2012

  4. Review

    Application of RNA silencing to plant disease resistance

    To reduce the losses caused by plant pathogens, plant biologists have adopted numerous methods to engineer resistant plants. Among them, RNA silencing-based resistance has been a powerful tool that has been us...

    Cheng-Guo Duan, Chun-Han Wang and Hui-Shan Guo

    Silence 2012 3:5

    Published on: 31 May 2012

  5. Short report

    Reducing ligation bias of small RNAs in libraries for next generation sequencing

    The use of nucleic acid-modifying enzymes has driven the rapid advancement in molecular biology. Understanding their function is important for modifying or improving their activity. However, functional analysi...

    Karim Sorefan, Helio Pais, Adam E Hall, Ana Kozomara, Sam Griffiths-Jones, Vincent Moulton and Tamas Dalmay

    Silence 2012 3:4

    Published on: 30 May 2012

  6. Minireview

    Noncoding RNA localisation mechanisms in chromatin regulation

    An important challenge in biology has been to understand how cell-type-specific expression programs are orchestrated through regulated access to chromatin. Knowledge of the interaction between noncoding RNAs (...

    Aditi Kanhere and Richard G Jenner

    Silence 2012 3:2

    Published on: 31 January 2012

  7. Review

    Inhibition of microRNA function by antimiR oligonucleotides

    MicroRNAs (miRNAs) have emerged as important post-transcriptional regulators of gene expression in many developmental and cellular processes. Moreover, there is now ample evidence that perturbations in the lev...

    Jan Stenvang, Andreas Petri, Morten Lindow, Susanna Obad and Sakari Kauppinen

    Silence 2012 3:1

    Published on: 9 January 2012

  8. Research

    Helicobacter pylori interferes with an embryonic stem cell micro RNA cluster to block cell cycle progression

    MicroRNAs, post-transcriptional regulators of eukaryotic gene expression, are implicated in host defense against pathogens. Viruses and bacteria have evolved strategies that suppress microRNA functions, result...

    Cédric Belair, Jessica Baud, Sandrine Chabas, Cynthia M Sharma, Jörg Vogel, Cathy Staedel and Fabien Darfeuille

    Silence 2011 2:7

    Published on: 25 October 2011

  9. Meeting report

    Noncoding RNAs and cancer

    The study of miRNAs and other noncoding RNAs has revolutionised our understanding of gene expression regulation during cancer development and progression, creating one of the fastest-growing research fields in...

    Ohad Yogev and Dimitris Lagos

    Silence 2011 2:6

    Published on: 29 September 2011

  10. Research

    Off-target effects dominate a large-scale RNAi screen for modulators of the TGF-β pathway and reveal microRNA regulation of TGFBR2

    RNA interference (RNAi) screens have been used to identify novel components of signal-transduction pathways in a variety of organisms. We performed a small interfering (si)RNA screen for novel members of the t...

    Nikolaus Schultz, Dina R Marenstein, Dino A De Angelis, Wei-Qing Wang, Sven Nelander, Anders Jacobsen, Debora S Marks, Joan Massagué and Chris Sander

    Silence 2011 2:3

    Published on: 14 March 2011

  11. Methodology

    In vivo quantification of formulated and chemically modified small interfering RNA by heating-in-Triton quantitative reverse transcription polymerase chain reaction (HIT qRT-PCR)

    While increasing numbers of small interfering RNA (siRNA) therapeutics enter into clinical trials, the quantification of siRNA from clinical samples for pharmacokinetic studies remains a challenge. This challe...

    Yosef Landesman, Nenad Svrzikapa, Armand Cognetta, Xuemei Zhang, Brian R Bettencourt, Satya Kuchimanchi, Keri Dufault, Sarfraz Shaikh, Maple Gioia, Akin Akinc, Renta Hutabarat and Rachel Meyers

    Silence 2010 1:16

    Published on: 23 August 2010

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