Figure 2

Sequence similarity between miR-21 and miR-122 is not sufficient to cause crossreactivity between inhibitors. (a) Diagram of possible pairing between (i) miR-122 mature (hsa-miR-122) and the miR-21 inhibitor target site, and (ii) miR-21 mature (hsa-mir-21) and the miR-122 inhibitor target site. The nucleotides in the seed region of the miRNA are indicated by shading. Solid lines represent Watson-Crick base pairs, and dotted lines represent G-U wobble base pairs. (b) Inhibitors targeting miR-21 and miR-122 were co-transfected with miR-21 or miR-122 reporters into Huh-7 cells 1 day after plating into 96-well plates, 10,000 cells/well, in antibiotic-free media. Inhibitor concentrations ranged from 0.2 to 25 nM, plasmid concentrations were constant at 100 ng/well. Dual-luciferase ratios were measured 2 days post-transfection. Results shown are averages from triplicate wells, normalized to appropriate controls, then expressed as fold-inhibition relative to no-inhibitor treatment. Error bars are ± 1SD (sample) of the original triplicate data, scaled for all subsequent calculations.