Figure 3

Mismatched hairpin inhibitor design to test sequence dependence of specificity. (a) Schematic showing miR-22 mature micro(mi)RNA sequence that is perfectly complementary to the central region of the hairpin inhibitor target site. (b) Sequences of mir-22 inhibitor target sites used in the mismatch study. Each inhibitor contained two consecutive mismatches (underlined) that are moved across the target site. Nucleotides were mismatched by substituting the reverse complement for the original nucleotide. Numbering of mismatches is from the 5' to the 3' end of the mature miRNA to which they would pair. The nucleotides that would pair with the seed region of the miRNA are indicated by shading.