Figure 1

Small interfering (si)RNA Lead selection. A large panel of siRNAs identified by a bioinformatic screen were synthesized and tested in vitro for activity against the transthyretin transcript as measured by quantitative PCR. The upper part of the panel shows the entire panel tested in parallel at a given nanomolar siRNA concentration, and data are shown in rank order of potency, with each vertical line representing an individual siRNA. The bottom panel illustrates in vitro dose response curves for a potent versus a less potent molecule.