Mutations in FBW2 suppress sqn and ago1 by increasing ARGONAUTE1 (AGO1). (A) Eighteen-day-old rosettes of WT, sqn-1, sqn-1 fbw2-1, and two independently isolated sqn-1 lines containing an AGO1::FLAG-AGO1 transgene. sqn-1 AGO1::FLAG-AGO1 transgenics resemble sqn fbw2 mutants. (B, C) Western blot of protein extracts from 14-day-old rosettes or floral buds probed with an anti-AGO1 antibody. Ponceau staining and a nonspecific band (*) were used as a loading control. (-) indicates samples that were slightly under-loaded.