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Figure 2 | Silence

Figure 2

From: In vivo quantification of formulated and chemically modified small interfering RNA by heating-in-Triton quantitative reverse transcription polymerase chain reaction (HIT qRT-PCR)

Figure 2

Amplification curves of modified antisense (AS) strands. AS strands were diluted in 0.25% Triton-phosphate-buffered saline (PBS), then were kept as indicated either at room temperature (25°C: AS 25C) or heated to 95°C for 10 min and added directly into reverse transcription reactions (AS 95C). The linear regression of the amplification curve for chemically modified AS at 95°C is shown (linear fit (AS 95C)). The average cycle threshold (AvCt) values indicate signals in single PCR reactions containing small interfering RNA (siRNA) equivalent concentrations ranging between 3.35 × 10-2 to 3.35 × 10-7 pmol. Each point is an average n = 3.

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ISSN: 1758-907X