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Figure 3 | Silence

Figure 3

From: In vivo quantification of formulated and chemically modified small interfering RNA by heating-in-Triton quantitative reverse transcription polymerase chain reaction (HIT qRT-PCR)

Figure 3

Reannealing of the modified duplex. The modified antisense (mod AS), modified duplex (mod Duplex) and lipid nanoparticle (LNP)-KC2-formulated duplex (KC2 mod Dup) small interfering RNAs (siRNAs) were diluted in 0.25% Triton-phosphate-buffered saline (PBS) and were first heated to 95°C for 10 min and then were kept for another 10 min at the indicated temperature before directly added into the RT reaction: (a) 95°C, (b) 85°C, (c) 75°C, (d) 25°C. The linear regression of the amplification curve for chemically modified AS is shown (linear fit (mod AS)). The average cycle threshold (AvCt) values indicate signals in single PCR reactions containing siRNA equivalent concentrations ranging between 3.35 × 10-2 to 3.35 × 10-7 pmol. Each point is an average n = 3.

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ISSN: 1758-907X