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Figure 4 | Silence

Figure 4

From: In vivo quantification of formulated and chemically modified small interfering RNA by heating-in-Triton quantitative reverse transcription polymerase chain reaction (HIT qRT-PCR)

Figure 4

Amplification curves of the modified antisense (mod AS), modified duplex (mod Duplex) and lipid nanoparticle (LNP)-KC2-formulated modified duplex (KC2 mod Duplex) in plasma and tissue. The small interfering RNAs (siRNAs) were diluted in 0.25% Triton-phosphate-buffered saline (PBS) and (a) 10% rat plasma or (b) 100 mg/ml frozen powdered rat liver lysate. Reverse transcription reactions were made from heated siRNA dilutions at 95°C. The linear regression of the amplification curves of formulated KC2 mod Duplex are shown (linear fit (KC2 mod Dup)). The average cycle threshold (AvCt) values indicate signals in single PCR reactions containing siRNA equivalent concentrations ranging between 3.35 × 10-3 to 3.35 × 10-7 pmol. Given the final plasma and liver tissue concentrations, the standard curves reveal dynamic ranges of at least four orders of magnitude: 0.7 μg to 70 pg siRNA per 1 ml of plasma or 1 g of liver tissue. Background Ct values (back) are indicated. Each point is an average n = 3.

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ISSN: 1758-907X