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Figure 5 | Silence

Figure 5

From: In vivo quantification of formulated and chemically modified small interfering RNA by heating-in-Triton quantitative reverse transcription polymerase chain reaction (HIT qRT-PCR)

Figure 5

Further examples of amplification curves of modified antisense (AS), modified duplex, and formulated modified duplex in plasma for the indicated small interfering RNAs (siRNAs). The modified siRNAs (a) AD1955 or (b) AD6490 were diluted in 0.25% Triton-phosphate-buffered saline (PBS)/10% rat plasma. Reverse transcription reactions were made from heated siRNA dilutions at 95°C. The linear regressions of the amplification curves of formulated KC2 or C12-200 modified duplexes are shown (linear fit (KC2 AD1955 or C12-200 AD6490)). The average cycle threshold (AvCt) values indicate signals in single PCR reactions containing siRNA equivalent concentrations ranging between 3.35 × 10-3 to 3.35 × 10-7 pmol. Background Ct values (back) are indicated. Each point is an average n = 3.

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ISSN: 1758-907X