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Figure 8 | Silence

Figure 8

From: In vivo quantification of formulated and chemically modified small interfering RNA by heating-in-Triton quantitative reverse transcription polymerase chain reaction (HIT qRT-PCR)

Figure 8

The detection of full-length, 5' and 3' truncated antisense oligonucleotides. (a) The 5'-truncated oligonucleotides (5' N-1 to 5' N-13) and (b) 3'-truncated oligonucleotides (3' N-1 to 3' N-6) were tested in 10-fold serial dilutions by the heating-in-Triton quantitative reverse transcription PCR (HIT qRT-PCR) method. The sequences of these oligonucleotides and their nomenclature are detailed in Table 2. The average cycle threshold (AvCt) values indicate signals in single PCR reactions containing small interfering RNA (siRNA)-equivalent concentrations ranging between 3.35 × 10-2 to 3.35 × 10-7 pmol. Each point is an average n = 3.

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