In vivo quantification of formulated and chemically modified small interfering RNA by heating-in-Triton quantitative reverse transcription polymerase chain reaction (HIT qRT-PCR)

Table 2 Set of 5' and 3' truncations of the modified antisense A4724.

Truncation name	Sequence 5'-3'
Full-length AS	GUfAAGACfUfUfGAGAUfGAUfCfCfdTsdT
5' N-1	UfAAGACfUfUfGAGAUfGAUfCfCfdTsdT
5' N-2	AAGACfUfUfGAGAUfGAUfCfCfdTsdT
5' N-3	AGACfUfUfGAGAUfGAUfCfCfdTsdT
5' N-4	GACfUfUfGAGAUfGAUfCfCfdTsdT
5' N-5	ACfUfUfGAGAUfGAUfCfCfdTsdT
5' N-6	CfUfUfGAGAUfGAUfCfCfdTsdT
5' N-7	UfUfGAGAUfGAUfCfCfdTsdT
5' N-8	UfGAGAUfGAUfCfCfdTsdT
5' N-9	GAGAUfGAUfCfCfdTsdT
5' N-10	AGAUfGAUfCfCfdTsdT
5' N-11	GAUfGAUfCfCfdTsdT
5' N-12	AUfGAUfCfCfdTsdT
5' N-13	UfGAUfCfCfdTsdT
3' N-1	GUfAAGACfUfUfGAGAUfGAUfCfCfdT
3' N-2	GUfAAGACfUfUfGAGAUfGAUfCfCf
3' N-3	GUfAAGACfUfUfGAGAUfGAUfCf
3' N-4	GUfAAGACfUfUfGAGAUfGAUf
3' N-5	GUfAAGACfUfUfGAGAUfGA
3' N-6	GUfAAGACfUfUfGAGAUfG

The full-length antisense (AS) strand sequence is indicated, along with the sequences of 5' (5' N-1 to 5' N-13) and 3' (3' N-1 to 3' N-6) truncated oligonucleotides. Chemical modifications are shown as follows: 2'-fluoro-modified nucleotides are shown by 'f' following the modified nucleotide and phosphorothioate linkages are shown by 's'. All sequences are written in the 5'-3' direction.