Figure 7
From: MicroR159 regulation of most conserved targets in Arabidopsis has negligible phenotypic effects
Expression of MYB101 and MYB120 is not regulated by miR159 in inflorescences. (A) The MYB101:GUS transgene consisted of 5171 bp of genomic sequence that included, 3.2 kb of 5' flanking region extending to the adjacent upstream gene (At2g32470), and 1.9 kb of the transcribed region of MYB101 (yellow arrow) that includes introns (black boxes) and the miR159 binding site (purple box) fused in frame to the β-glucuronidase (GUS) reporter gene to encode a full length MYB101:GUS translational fusion protein. Eight synonymous nucleotide substitutions were made in the miR159 binding site of MYB101:GUS to generate mMYB101:GUS. Figure is not to scale. (B) GUS staining of the inflorescence of a MYB101:GUS transgenic plant. (C) GUS staining of the inflorescence of a mMYB101:GUS transgenic plant. (D) The MYB120:GUS transgene consisted of 3125 bp of genomic sequence that included, 1.5 kb of 5' flanking region and 1.6 kb of the coding region of MYB120 (yellow arrow) that includes an intron (black box) and the miR159 binding site (purple box) fused in frame to the GUS reporter gene to encode a full length MYB120:GUS translational fusion protein. Seven synonymous nucleotide substitutions were made in the miR159 binding site of MYB120:GUS to generate the MYB120:GUS transgene. Figure is not to scale. (E) GUS staining of the inflorescence of a MYB120:GUS transgenic plant. (F) GUS staining of the inflorescence of a mMYB120:GUS transgenic plant.