Figure 1
From: Inhibiting miRNA in Caenorhabditis elegans using a potent and selective antisense reagent
Inhibit miRNA in Caenorhabditis elegans using a dextran-conjugate of antisense 2'- O -methyl oligoribonucleotide. (a) Chemical synthesis of dextran-(as-2'OMelin-4)8, or D-(as-2'OMelin-4)8, a dextran conjugate of an antisense reagent against lin-4. (b) Dose response of D-(as-2'OMelin-4)8 in causing egg laying defectives (Egl) via lin-4 inhibition. Controls include as-2'OMelin-4, a lin-4 antisense 2'-O-methyl oligoribonucleotide without dextran; D-(s-2'OMelin-4)8 and D-(as-2'OMemiR-237)8, dextran conjugates containing either lin-4 sense or miR-237 antisense 2'-O-methyl oligoribonucleotide. Error bars are standard errors of two independent injections. Each time ~ eight worms were injected and 50 or more labelled embryos from injected worms were scored for Egl when they reached adults. (c, d) Example images of a normal worm (c) and an Egl worm (d) labelled with dextran-(as-2'OMelin-4)8.