Figure 11
From: Inhibiting miRNA in Caenorhabditis elegans using a potent and selective antisense reagent
Concurrent inhibition of lin-4 and lsy-6 in Caenorhabditis elegans with two antisense reagents. (a) Rhdextran-(as-2'OMelin-4)1 (50 μM) or Rhdextran-(as-2'OMelsy-6)1 (20 μM), or a mixture of both reagents, were injected into gonads of OH3192 strain (gcy-5prom::gfp). The egg laying defective (Egl) and ectopic expression of gcy-5prom::gfp in ASEL (left ASE) were scored (n = 50 worms. Error bars are standard errors of two independent injection experiments). (b, c) Bright field [(b) showing Egl] and fluorescence [(c) showing ectopic expression of gcy-5prom::gfp in ASEL] images of a worm (OH3192) labelled with both Rhdextran-(as-2'OMelin-4)1 and Rhdextran-(as-2'OMelsy-6)1.