Figure 4
From: Inhibiting miRNA in Caenorhabditis elegans using a potent and selective antisense reagent
lin-4 inhibition with Rhdextran-(as-2'OMelin-4 ) 1 disrupted vulva formation similarly as in a lin-4(0) mutant. The vulval structures in mid-L4 (top) and in adult (bottom) are well defined in control animals (a). Vulva failed to form in lin-4(e912) (b) and in lin-4 knockdown (c). The penetrance of vulval defect in (c) was 100% (n = 45) with 50 μM of Rhdextran-(as-2'OMelin-4)1.