Figure 6
From: Inhibiting miRNA in Caenorhabditis elegans using a potent and selective antisense reagent
lin-4 knockdown caused repetition of L2 seam cell division pattern in L3. (a) Seam cell lineage for V1 - V4 and V6 of wild type, lin-4(0) and lin-4 knockdown by Rhdextran-(as-2'OMelin-4)1 at 50 μM injection concentration. (b, c) In early L2, each N2 seam cell gave rise to a quartet of daughter cells after two successive divisions in both N2 (b) and in lin-4 knockdown (c). (d, e) In early L3, each Vn cell of lin-4 knockdown repeated L2 programme, giving rise to twice as many daughter cells (e) as those of control animals (d). Each image is representative of at least six animals (see text). Scale bar = 20 μm.