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Figure 1 | Silence

Figure 1

From: Effect of small interfering RNA 3'-end overhangs on chemosensitivity to thymidylate synthase inhibitors

Figure 1

Comparison of Lipofectamine 2000 (LF2000) and polyethylenimine (PEI) for siRNA transfection. RKO cells were transfected with TS6 siRNAs (100 nM) complexed either LF2000 (lanes 1-3) or linear jetPEI (lanes 4-6). After 48 h, cells were harvested and processed for western blot analysis as described in the Methods section. Lane 1 contains cell lysates from LF2000-treated cells; lane 2, TS6 siRNA; lane 3, TS6 mismatch siRNA; lane 4, TS6 siRNA; lane 5, TS6 sticky end siRNA (ssiRNA); lane 6, TS6 mismatch ssiRNA.

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Silence

ISSN: 1758-907X