Figure 3
Effect of ssiRNA/siRNA transfection on TS protein and mRNA expression. RKO cells were transfected with TS6 siRNAs using LF2000. After 48 h, cells were harvested for western blot (a) or qPCR analysis (b) as described in the Methods section. (a) Representative western blot from three similar experiments. The percentage TS protein knockdown was quantified after scanning and analysis by ImageJ software. (b) Relative mRNA levels after siRNA transfection. Values represent the mean ± standard deviation (SD) from three individual experiments analyzed in triplicate. TS/18S values were normalized to the value of cells treated with LF2000 alone, which was set to 100.