Figure 4

Effect of RNA/ssiRNA/siRNA transfection on ZD1694 toxicity. RKO cells were exposed to 3 nM ZD1694 (TDX) with or without (a) RNA, (b) ssiRNA, or (c) siRNA transfection. After 96 h, cell growth was determined by the WST-1 assay. Untreated cells (LF2000 alone) were normalized to 100%. Values represent the mean ± standard error of the mean (SEM) from at least four separate experiments performed in duplicate. *P values < 0.05 versus ZD1694 treatment alone.