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Table 1 Small interfering RNA (siRNA) sequences

From: Effect of small interfering RNA 3'-end overhangs on chemosensitivity to thymidylate synthase inhibitors

siRNA

 

Sequence

TS6-dTdT

5'-

GGAUAUUGUCAGUCUUUAGG-dTdT-'3

 

3'-dTdT-

CCUAUAACAGUCAGAAAUCC-'5

TS6-dUdU

5'-

GGAUAUUGUCAGUCUUUAGG-dUdU-'3

 

3'-dUdU-

CCUAUAACAGUCAGAAAUCC-'5

TS6-dTx5

5'-

GGAUAUUGUCAGUCUUUAGG-dTdTdTdTdT-'3

 

3'-dAdAdAdAdA-

CCUAUAACAGUCAGAAAUCC-'5

TS6-dUx5

5'-

GGAUAUUGUCAGUCUUUAGG-dUdUdUdUdU-'3

 

3'-dAdAdAdAdA-

CCUAUAACAGUCAGAAAUCC-'5

Mismatch-dTx5

5'-

GGAUACUGCCAAUCUCUAGG-dTdTdTdTdT-'3

 

3'-dAdAdAdAdA-

CCUAUGACGGUUAGAGAUCC-'5

  1. These siRNAs target nucleotides 1106-1123 of human thymidylate synthase (TS) mRNA. The top strand is the sense (passenger) strand with the bottom strand being the antisense (guide) strand of the siRNA. Mismatches are shown in bold type.
  2. dA = deoxyadenosine; dT = deoxythymidine; dU = deoxyuridine;.