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Figure 2 | Silence

Figure 2

From: Experimental design, preprocessing, normalization and differential expression analysis of small RNA sequencing experiments

Figure 2

Sample file formats for small RNA libraries. Illumina machines generate .bcl files, which are in binary form and are not human-readable. These files are converted into .qseq files, which record the most likely sequence and a quality score for each read. Scripts are available to convert files in .qseq format into .fastq or SCARF format (Solexa Compact ASCII Read Format). Files in these formats are often converted to a "tag count" format so that they can be easily stored and analyzed.

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ISSN: 1758-907X