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Figure 5 | Silence

Figure 5

From: Experimental design, preprocessing, normalization and differential expression analysis of small RNA sequencing experiments

Figure 5

Example of two-step nonlinear normalization. An example of the normalization process applied to the binding quantity difference regarding breast cancer data on human chromosome 1 between (1) MCF-7 control and (2) MCF-7 with E2 stimulation. (A) Raw data with clear bias toward the positive direction. (B) Data normalized with respect to the mean. (C) Data normalized with respect to both mean and variance (x-axis is zoomed in). Green dashed-dotted line and magenta dashed line represent the locally weighted linear regression line with respect to the mean and variance, respectively. Red dotted line represents the zero difference line. Reproduced with permission from Oxford University Press from Taslim et al. [156].

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