Skip to main content
Figure 1 | Silence

Figure 1

From: Off-target effects dominate a large-scale RNAi screen for modulators of the TGF-β pathway and reveal microRNA regulation of TGFBR2

Figure 1

Results of a 6,000 gene small interfering (si)RNA screen using a green fluorescent protein (GFP)-SMAD2 translocation assay. (A) GFP-SMAD2 translocation assay in the context of the transforming growth factor (TGF)-β pathway. (B) Screen timeline. (C) Distribution of normalized nuclear:cytosolic (N:C) ratios for all siRNAs used in the screen. Screen 'hits' show increased or decreased GFP-SMAD2 nuclear translocation relative to the mean after TGF-β stimulation. The N:C ratio was determined by image analysis of an average of 220 cells per well. Group 1 comprised 134 small interfering (si)RNAs that led to a decrease in GFP-SMAD2 translocation (σ > 2.5), whereas group 2 comprised 42 siRNAs that led to an increase in GFP-SMAD2 translocation (σ > 2.5).

Back to article page

Silence

ISSN: 1758-907X