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Figure 3 | Silence

Figure 3

From: Off-target effects dominate a large-scale RNAi screen for modulators of the TGF-β pathway and reveal microRNA regulation of TGFBR2

Figure 3

Confirmation that screen small interfering (si)RNA hits acted primarily on the 3' untranslated region (UTR) of the transforming growth factor-β receptor 2 (TGFBR2) mRNA. Silencing of luciferase-TGFBR2 3' UTR or luciferase TGFBR2 open reading frame (ORF) protein expression by four representative siRNA hits. Two independent siRNAs were used as non-targeting controls (controls 1 and 2), and a microRNA miR-20a mimic and an siRNA with perfect complementarity to the TGFBR2 ORF were used as positive controls for the 3' UTR and the ORF constructs, respectively. The data are reported (y-axis) as the relative repression of firefly luciferase expression standardized to Renilla luciferase as a transfection control. Integers (for example, 9, 18) label ranked siRNA hits. Error bars on each column are the mean ± SD of three experiments.

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Silence

ISSN: 1758-907X