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Figure 4 | Silence

Figure 4

From: Off-target effects dominate a large-scale RNAi screen for modulators of the TGF-β pathway and reveal microRNA regulation of TGFBR2

Figure 4

On-target and off-target mechanisms of small interfering (si)RNAs. All siRNAs were designed to have perfect complementarity between the siRNA guide strand and the open reading frame (ORF) of the target gene. Off-target effects were mediated primarily through partial complementarity with the 3' untranslated region (UTR) of off-target genes. Complementarity of the siRNA seed (positions 2 to 8), possibly containing G:U wobbles, was thought to be the key determinant Additional known features were the occurrence of an A in the mRNA opposite position 1 of the siRNA (t1A), and partial pairing between the 3' end of the siRNA and the mRNA.

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