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Table 1 Repression of TGFBR1 and TGFBR2 mRNAs by the 193 tested screen siRNAa hitsb.

From: Off-target effects dominate a large-scale RNAi screen for modulators of the TGF-β pathway and reveal microRNA regulation of TGFBR2

Off-target mRNAs affected Number of siRNAs
TGFBR1 only 21c
TGFBR2 only 109
Both TGFBR1 and TGFBR2 42
No off-target effect (false positives) 21
  1. asiRNA = small interfering RNA.
  2. bScreen hits were grouped by their effects on mRNA levels of TGFBR1 and TGFBR2, as determined by branched DNA assay (most siRNA) or quantitative (q)PCR (some siRNA).
  3. cFor the 21 siRNAs without off-target effects, the effects on TGF-β signaling were not verifiable by phospho-SMAD2 western blots or by qPCR of characteristic TGF-β target gene mRNAs (false positives) (see Additional File 2 Table S3 for detailed expression data).