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Figure 3 | Silence

Figure 3

From: A 5′-uridine amplifies miRNA/miRNA* asymmetry in Drosophilaby promoting RNA-induced silencing complex formation

Figure 3

Identity of the first miRNA nucleotide affects duplex asymmetry. RNA-induced silencing complex (RISC) loading of miRNA and miRNA* strands was measured after in vitro assembly. Data are reported as means ± standard deviation for three independent replicate experiments. (A) Swapping the terminal uridine:adenosine (U:A) pair of the miR-2a/miR-2a-1* duplex decreased miRNA loading and increased miRNA* loading. (B) The effect of the terminal A:U pair mainly reflects the identity of the first miRNA nucleotide, which affects both miRNA and miRNA* loading (C), whereas the identity of the facing miRNA* nucleotide has no detectable effect (D).

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