Table 1 Synthetic oligonucleotides used in this study^a

miR-2a with P1 U

UAU CAC AGC CAG CUU UGA UGA GC

miR-2a with P1 A

AAU CAC AGC CAG CUU UGA UGA GC

miR-2a with P1 I

IAU CAC AGC CAG CUU UGA UGA GC

miR-2a with P1 C

CAU CAC AGC CAG CUU UGA UGA GC

miR-2a with P1 G

GAU CAC AGC CAG CUU UGA UGA GC

miR-2a-1* with P19 A

UCU CAA AGU GGU UGU GAA AUG

miR-2a-1* with P19 U

UCU CAA AGU GGU UGU GAA UUG

miR-2a-1* with P19 C

UCU CAA AGU GGU UGU GAA CUG

miR-2a-1* with P19 I

UCU CAA AGU GGU UGU GAA IUG

miR-184 with P1 U

UGG ACG GAG AAC UGA UAA GGG C

miR-184 with P1 A

AGG ACG GAG AAC UGA UAA GGG C

miR-184 with P1 C

CGG ACG GAG AAC UGA UAA GGG C

miR-184 with P1 T

TGG ACG GAG AAC UGA UAA GGG C

miR-184 with P1 G

GGG ACG GAG AAC UGA UAA GGG C

miR-184* with P19

CCU UAU CAU UCU CUC GCC CCG

miR-184* with P19

CCU UAU CAU UCU CUC GCC ACG

miR-184* with P21

CCU UAU CAU UCU CUC GCC CCC

miR-184* with P21 U

CCU UAU CAU UCU CUC GCC CCU

miR-184* with P21 A

CCU UAU CAU UCU CUC GCC CCA

miR-14 with P1 U

UCA GUC UUU UUC UCU CUC CUA

miR-14* with P1 A

GGA GCG AGA CGG GGA CUC ACU

miR-14 with P1 A

ACA GUC UUU UUC UCU CUC CUA

miR-14* with P19 U

GGA GCG AGA CGG GGA CUC UCU

miR-2c with P1 U

UAU CAC AGC CAG CUU UGA UGG GC

miR-2c* with P20 A

CAU CAA AAA GGG CUG AAG AAA G

Oligo to capture miR-2a and miR-2c

Bio-mAmUmGmU mUmGmG mCmUmC mAmUmC mAmAmA mGmCmU mGmGmC mUmGmU mGmAmU mCmUmG mCmUmG mA

Oligo to capture miR-2a-1*

Bio-mAmUmG mUmUmG mCmAmC mUmUmC mAmCmA mAmCmC mAmCmU mUmUmG mAmGmA mUmGmC mUmGmA

Oligo to capture miR-184

Bio-mAmUmG mUmUmG mGmCmC mCmUmU mAmUmC mAmGmU mUmCmU mCmCmG mUmCmC mCmUmG mCmUmG mA

Oligo to capture miR-184*

Bio-mAmUmG mUmUmG mCmGmG mGmGmC mGmAmG mAmGmA mAmUmG mAmUmA mAmGmG mUmGmC mUmGmA

Oligo to capture miR-14

Bio-mAmUmG mUmUmG mUmAmG mGmAmG mAmGmA mGmAmA mAmAmA mGmAmC mUmGmC mUmGmC mUmGmA

Oligo to capture miR-14*

Bio-mAmUmG mUmUmG mAmGmC mGmAmG mUmCmC mCmCmG mUmCmU mCmGmC mUmCmC mUmGmC mUmGmA

Oligo to capture miR-2c*

Bio-mAmUmG mUmUmG mCmUmU mUmCmU mUmCmA mGmCmC mCmUmU mUmUmU mGmAmU mGmUmG mCmUmG mA

pre-miR-2a-1 loop (extended by 4 nt)

CAU UUC CGC UUU GCG CGG CAU AUC

miR-2a (shortened by 4 nt)

ACA GCC AGC UUU GAU GAG C

DNA splint for pre-miR-2a-1 ligation

GCT AAG CTC ATC AAA GCT GGC TGT GAT ATG CCG CGC AAA GCG GAA ATG CAT TTC ACA ACC ACT TTG AGA GCT TA

pre-miR-2a-1

UCU CAA AGU GGU UGU GAA AUG CAU UUC CGC UUU GCG CGG CAU AUC ACA GCC AGC UUU GAU GAG C

miR-2a with U at position 1 and U at position 2

UUU CAC AGC CAG CU UUG AUG AGC

miR-2a with A at position 1 and U at position 2

AUU CAC AGC CAG CUU UGA UGA GC

miR-2a with C at position 1 and U at position 2

CUU CAC AGC CAG CUU UGA UGA GC

miR-184 with U at position 1 and C at position 2

UCG ACG GAG AAC UGA UAA GGG C

miR-184 with A at position 1 and C at position 2

ACG ACG GAG AAC UGA UAA GGG C

miR-184 with C at position 1 and C at position 2

CCG ACG GAG AAC UGA UAA GGG C