Figure 1From: Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis thalianaStructure of LUCH and its neighboring transgene. RB and LB, right border and left border of the T-DNA, respectively. The arrows indicate the directions of the coding regions. The d35S fragments (marked #1 to #3) specific for the d35S promoter upstream of LUC are amplified by PCR following digestion with the restriction enzyme McrBC as well as in bisulfite sequencing.Back to article page