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Figure 3 | Silence

Figure 3

From: Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis thaliana

Figure 3

The RdDM pathway is genetically required for the suppression of LUCH expression. (A) De-repression of LUC luminescence in various RdDM mutants. Each spot represents an Arabidopsis seedling. The brighter the spots, the higher the LUC luminescence. Col-0, wild type (with no transgene). (B) RT-PCR of LUC and NPTII in various RdDM mutants. UBQ5 serves as a loading control. RT (−), UBQ5 RT-PCR in which the reverse transcription was conducted in the absence of the reverse transcriptase. (C) Analysis of cytosine methylation in the d35S in LUCH in ago4–6, ros1–5 and mom1–5 mutants by McrBC-PCR. ACT1 serves as an internal, unmethylated control.

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Silence

ISSN: 1758-907X