Figure 3
From: Generation of a luciferase-based reporter for CHH and CG DNA methylation inArabidopsis thaliana
met1-3 releases DNA methylationin LUCL . (A) Luciferase luminescence ofLUCL and LUCL met1-3. The top panel contains twoLUCL seedlings and the bottom panel contains two LUCLmet1-3 seedlings. (B) RT-PCR of LUC transcriptlevels. UBQ5 was used as an internal control. (C)Bisulfite sequencing analyses of LUCL (blue bars) and LUCLmet1-3 (red bars) reveal that CG methylation is reduced at allfour regions tested in LUCL met1-3. The regions tested areindicated in Figure 2D. RT-PCR: reversetranscription-PCR.