Figure 2

Relative Gus activity of Fusarium oxysporum lines containing the Gus gene. Gus activity was measured by MUG assays of at least three biological replicates of each line. The Y-axis depicts Gus activity per μg of protein extract, while each column on the X-axis represents the activity of a single transgenic line. Standard deviations are indicated on each column. The number of Gus T-DNA insertions present in each line is indicated below the X-axis and was determined by DNA blot hybridization using a probe specific for the hygromycin selectable marker gene. Lines 0–1.3, 0–1.6 and 1–1.3 were used for subsequent hpGus transformation.