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Table 3 Cleavage products obtained by 5′ RACE of RNA samples from line S5: Gus W4

From: Analysis of hairpin RNA transgene-induced gene silencing in Fusarium oxysporum

Cleavage site inGusmRNA (nt)c

Number of fragments sequencedd

80 | 81b

3

428 | 429b

5

548 | 549b

7

587 | 588a

6

623 | 624a

3

778 | 779a

1

  1. Gus, β-glucuronidase, nt nucleotides.
  2. aNucleotides 556–792 correspond to the unique region of the Gus gene. bThe first 555 nucleotides of the Gus gene were used to construct the double stranded arm region of the hpGus gene. cShown are the nucleotide (nt) numbers of the Gus gene between which cleavage had occurred and dhow often this fragment was recovered.
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Silence

ISSN: 1758-907X