Figure 1From: Effect of small interfering RNA 3'-end overhangs on chemosensitivity to thymidylate synthase inhibitorsComparison of Lipofectamine 2000 (LF2000) and polyethylenimine (PEI) for siRNA transfection. RKO cells were transfected with TS6 siRNAs (100 nM) complexed either LF2000 (lanes 1-3) or linear jetPEI (lanes 4-6). After 48 h, cells were harvested and processed for western blot analysis as described in the Methods section. Lane 1 contains cell lysates from LF2000-treated cells; lane 2, TS6 siRNA; lane 3, TS6 mismatch siRNA; lane 4, TS6 siRNA; lane 5, TS6 sticky end siRNA (ssiRNA); lane 6, TS6 mismatch ssiRNA.Back to article page