A 5′-uridine amplifies miRNA/miRNA* asymmetry in Drosophilaby promoting RNA-induced silencing complex formation

Silence

Table 2 High throughput sequencing data used in this study^a

Species	Accession Number
Caenorhabditis elegans	GSM139137, GSM297742, GSM297743, GSM297744, GSM297745, GSM297746, GSM297747, GSM297748, GSM297750, GSM297751
Drosophila melanogaster	GSM180328, GSM180329, GSM180330, GSM180331, GSM180332, GSM180333, GSM180334, GSM180335, GSM180336, GSM180337, GSM239041, GSM239052, GSM239054, GSM239056, GSM240749, GSM246084, GSM272651, GSM272652, GSM272653, GSM275691, GSM280082, GSM280085, GSM286602, GSM286603, GSM286604, GSM286605, GSM286606, GSM286607, GSM286611, GSM286613, GSM322208, GSM322219, GSM322245, GSM322338, GSM322533, GSM322543, GSM343832, GSM343833, GSM360256, GSM360257, GSM360260, GSM360262, GSM361908, GSM364902, GSM371638, GSM385744, GSM385748, GSM385821, GSM385822, GSM399100, GSM399101, GSM399105, GSM399106, GSM399107, GSM399110, GSM609217, GSM609218, GSM609219, GSM609220, GSM609221, GSM609222, GSM609223, GSM609224, GSM609225, GSM609226, GSM609227, GSM609228, GSM609229, GSM609234, GSM609235, GSM609238, GSM609239, GSM609240, GSM609241, GSM609242, GSM609243, GSM609244, GSM609246, GSM609247, GSM609248, GSM609249, GSM609250, GSM609251
Mus musculus	GSM237107, GSM237110, GSM261957, GSM261959, GSM304914, GSM314552, GSM314558

^aDatasets were obtained from the GEO database (http://www.ncbi.nlm.nih.gov/geo/). Reads (18 to 30 nt) matching known miRNA or miRNA sequences were identified, tolerating up to 9 nt between the observed extremities and the 5′ and 3′ extremities described in miRBase version 14. Overall, the fly libraries contained 100,603,194 miRNA and 6,569,021 miRNA* reads, the worm libraries contained 14,479,717 miRNA and 1,124,773 miRNA* reads and the mouse libraries contained 3,416,073 miRNA and 143,617 miRNA* reads.