Figure 1

Schematic diagrams (not to scale) of transgenes introduced into Fusarium oxysporum . All Gus-derived sequences are shown in black, except a small region of the Gus ORF represented in grey, which is present in the full length Gus gene but absent in the hpGus constructs. The gpdA promoter is shown as an open arrow, while the transcription terminator sequence trpC is indicated as an open box. Sequences present in hairpin constructs of the Gfp and F. oxysporum Frp1 are shown as light grey regions in each construct, respectively. An intron from the pyruvate dehydrogenase kinase gene (pdk) is included in the hpGfp gene. The hygromycin phosphotransferase gene (hyg) and Streptomyces verticillius bleomycin gene (phle) were used as selectable markers for F. oxysporum transformation and are shown as dark grey arrows. The positions and expected fragment sizes of restriction endonuclease recognition sites used for DNA blot analyses are indicated.